MLS 300. Introduction to Molecular Diagnostics

Credits: 2

This course offers fundamentals of clinical diagnosis and management of disease by molecular biology laboratory methods. Disorders due to inherited or acquired molecular defects such as errors of metabolism, hemoglobinopathies, leukemia, and cystic fibrosis are discussed. Principles and procedures for the diagnosis and management of infectious diseases by the molecular methods are also included. The discussion of molecular approaches to diagnosing and monitoring these diseases will span the conventional methods of PCR, gel electrophoresis and Southern Blotting to semi-automated methods of TMA, LCR and Real-time PCR. A survey of molecular diagnostic methods currently available in various sections of a clinical laboratory is included. The laboratory component will involve methods for specimen collection and handling, contamination control, amplification and detection of genetic material from humans and microorganisms. Methodologies include conventional PCR, electrophoresis for DNA and proteins, real time PCR, densitometry, Southern Blot and Western Blot techniques.
Course Fee: $80.00
Offered: On Campus